S. Kuphal, L. Vardimon, Melanie Kappelmann-Fenzl, S. Meierjohann, B. Spangler, B. Schittek, A. Bosserhoff
ETS‐1/RhoC signaling regulates the transcription factor c‐Jun in melanoma
International Journal of Cancer, vol. 130, no. 12, pp. 2801-2811
Recently, we discovered that the loss of E‐cadherin induces c‐Jun protein expression, which is a member of the AP‐1 transcription factor family and a key player in the processes of cell proliferation and tumor development and also found in elevated levels in melanomas. Notably, the mRNA level of c‐Jun was not affected, suggesting that c‐Jun is regulated at post‐transcriptional level. Here, we present data that suggest that the dynamic cytoskeletal network, linked to E‐cadherin, is involved in the regulation of the c‐Jun protein and transcriptional activity. In a signaling cascade, the loss of E‐cadherin activates the transcriptional regulator ETS‐1 and consequently leads to the induction of RhoC expression that stabilizes c‐Jun in melanoma. The link between RhoC and c‐Jun seems to be indirect via the cytoskeleton. We conclude that the loss of E‐cadherin mediated cell‐adhesion induces c‐Jun protein expression in a multistep process, offering several possibilities for therapeutic intervention.
Elektrotechnik und MedientechnikHochschulleitung und -einrichtungen
A. Penzkofer, W. Spangler, Peter Sperber, B. Meier
Experimental and theoretical investigation of tunable picosecond pulse generation in longitudinally pumped dye laser generators and amplifiers
Optical and Quantum Electronics, vol. 20, pp. 395-431
Picosecond pulse generation in longitudinally pumped dye laser generators and amplifiers is studied experimentally and theoretically. Frequency-tunable pulses between 720 and 940 nm are generated with a picosecond ruby laser pump source. The amplification of spontaneous emission and of seeding pulses in the generator and amplifier cells is investigated. Stimulated emission cross-sections and excited-state absorption cross-sections are determined by computer simulations. The coherence properties of the generated radiation are analysed. Resonance Raman contributions are resolved.